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Flook D
Professor Dave Explains
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Comments by "Flook D" (@flookd5516) on "Gel Electrophoresis" video.
EtBr will (with UV) but bromophenol blue is just a marker dye that tells you the gel is running OK.
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You can extract DNA/RNA from a cut-out section of an agarose gel by dissolving it.
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3:05 & 3:15. The left hand lane contains DNA of fixed sizes to use as a scale. In checking correct insertion you can run a lane with the unaltered plasmid as a comparison or (better) digest some of the new plasmid and check to see if the right size insert appears.
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Non-denaturing PAGE.
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